Hotspot

rbcL-psaI

LSC · 56681-58553 · 1873 bp

Back to Zizania

Pi 0.00925

MarkerSeek score 37.00

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1873
variable_sites: 26
pi: 0.00925
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	2245-2247 bp	1.0	81.0
rbcL-psaI_p2	TTGCAACTGGAGTCCTGAAC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2032-2034 bp	1.0	80.9
rbcL-psaI_p3	GAGCTGTATTTGCGAGGGAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	2618-2620 bp	1.0	80.7
rbcL-psaI_p4	GTTATACCGGTGGCTTCAGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	2256-2258 bp	1.0	80.7
rbcL-psaI_p5	GCATGACTACTTAACCGGGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	2578-2580 bp	1.0	80.5