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rps4-trnT-UGU

LSC · 46619-46944 · 326 bp

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Pi 0.00833

MarkerSeek score 54.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 4
pi: 0.00833
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CGAGGGTCATCGGTTCAAAT	50.0	50.0	58.3	58.0	402-408 bp	1.0	79.7
rps4-trnT-UGU_p2	CTCCCAGACGGCGTATTTTT	TTGTAATGCGAGGGTCATCG	50.0	50.0	58.3	58.1	410-416 bp	1.0	79.3
rps4-trnT-UGU_p3	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	432-438 bp	1.0	78.7
rps4-trnT-UGU_p4	GTGAGTCCTGGTAAAGCTCC	CGAGGGTCATCGGTTCAAAT	55.0	50.0	57.6	58.0	418-424 bp	1.0	78.6
rps4-trnT-UGU_p5	GTGAGTCCTGGTAAAGCTCC	TTGTAATGCGAGGGTCATCG	55.0	50.0	57.6	58.1	426-432 bp	1.0	78.2