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trnD-GUC-psbM

LSC · 16379-17310 · 932 bp

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Pi 0.00858

MarkerSeek score 59.40

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 12
pi: 0.00858
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	50.0	45.0	58.5	55.8	1043-1047 bp	1.0	59.4
trnD-GUC-psbM_p2	TAGGTATGCCATACACCCCG	AGAATGAACAGTGCAGTAGC	55.0	45.0	58.7	55.8	1075-1079 bp	1.0	58.0
trnD-GUC-psbM_p3	TTCAATTGGTCAGAGCACCG	AGGAACTAGAATGAACAGTGCA	50.0	40.9	58.5	57.4	1050-1054 bp	1.0	56.3
trnD-GUC-psbM_p4	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.0	55.8	1044-1048 bp	1.0	55.3
trnD-GUC-psbM_p5	TAGGTATGCCATACACCCCG	AGGAACTAGAATGAACAGTGCA	55.0	40.9	58.7	57.4	1082-1086 bp	1.0	54.8