Hotspot

ycf2

IRb · 91064-97933 · 6870 bp

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Pi 0.00198

MarkerSeek score 32.60

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6870
variable_sites: 77
pi: 0.00198
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 32.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CGCAACAAATGGAGGAGGAT	TCTGCAAGAACTCCTCCTCA	50.0	50.0	58.2	58.0	177-297 bp	1.0	84.7
ycf2_p2	AGAGGAAGAGGACATCGACC	TCTGCAAGAACTCCTCCTCA	55.0	50.0	58.2	58.0	197-317 bp	1.0	84.6
ycf2_p3	CGCAACAAATGGAGGAGGAT	GTTCCCCTCTGCAAGAACTC	50.0	55.0	58.2	58.2	184-304 bp	1.0	83.2
ycf2_p4	AGAGGAAGAGGACATCGACC	GTTCCCCTCTGCAAGAACTC	55.0	55.0	58.2	58.2	204-324 bp	1.0	83.1
ycf2_p5	AGAGGAAGAGGACATCGACC	ACTCCTCCTCATGCTCTTGA	55.0	50.0	58.2	57.8	188-308 bp	1.0	82.8