Hotspot

ycf1

IRa · 132223-137679 · 5457 bp

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Pi 0.0124

MarkerSeek score 55.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 5457
variable_sites: 390
pi: 0.0124
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTACGTCTTTGAGCGCGTAA	ACTAAACAGGAACAAGCGGG	45.0	50.0	58.0	58.1	1223-1253 bp	0.956522	84.5
ycf1_p2	TTTACGTCTTTGAGCGCGTA	ACTAAACAGGAACAAGCGGG	45.0	50.0	58.0	58.1	1224-1254 bp	0.956522	84.5
ycf1_p3	TCTTTGAGCGCGTAAGGATC	ACTAAACAGGAACAAGCGGG	50.0	50.0	58.1	58.1	1218-1248 bp	0.956522	84.3
ycf1_p4	AGCGTGGATCAGGAGTATCA	ACTAAACAGGAACAAGCGGG	50.0	50.0	57.9	58.1	1545-1575 bp	0.869565	76.7
ycf1_p5	GGGATTCACGCTGTAGTCAG	ACTAAACAGGAACAAGCGGG	55.0	50.0	58.1	58.1	1116 bp	0.826087	71.9