Hotspot

ycf2-trnI-CAU

IRa · 160770-160948 · 179 bp

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Pi 0.0442

MarkerSeek score 75.20

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 179
variable_sites: 40
pi: 0.0442
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	CTCAATTCGACGATCCAGGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.1	58.0	202-594 bp	1.0	85.0
ycf2-trnI-CAU_p2	TCTCAATTCGACGATCCAGG	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.1	58.0	203-595 bp	1.0	85.0
ycf2-trnI-CAU_p3	CTCAATTCGACGATCCAGGA	AGTTGGGCGCTTTAACCATT	50.0	45.0	57.1	58.4	203-595 bp	1.0	82.1
ycf2-trnI-CAU_p4	TCTCAATTCGACGATCCAGG	AGTTGGGCGCTTTAACCATT	50.0	45.0	57.1	58.4	204-596 bp	1.0	82.1
ycf2-trnI-CAU_p5	CTCAATTCGACGATCCAGGA	TTGGGCGCTTTAACCATTCA	50.0	45.0	57.1	58.4	201-593 bp	1.0	82.0