Hotspot

psaC-ndhE

SSC · 125318-126018 · 701 bp

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Pi 0.0359

MarkerSeek score 77.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 701
variable_sites: 130
pi: 0.0359
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGTCCCACGGTATCATTTCC	AACGCGTATCAACCAATCGA	55.0	45.0	58.4	58.0	675-871 bp	1.0	88.6
psaC-ndhE_p2	CGTCCCACGGTATCATTTCC	ACGCGTATCAACCAATCGAA	55.0	45.0	58.4	58.0	674-870 bp	1.0	88.6
psaC-ndhE_p3	CGTCCCACGGTATCATTTCC	CAACGCGTATCAACCAATCG	55.0	50.0	58.4	57.9	676-872 bp	1.0	88.1
psaC-ndhE_p4	GGCAAGCTCGTACACATTGA	AACGCGTATCAACCAATCGA	50.0	45.0	58.6	58.0	642-838 bp	1.0	87.5
psaC-ndhE_p5	GGCAAGCTCGTACACATTGA	ACGCGTATCAACCAATCGAA	50.0	45.0	58.6	58.0	641-837 bp	1.0	87.5