Hotspot

ccsA-ndhD

SSC · 123166-123440 · 275 bp

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Pi 0.0295
MarkerSeek score 62.80
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
275
variable_sites
37
pi
0.0295
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGTGGCTTCTATGGGATTCC TCAGTTGATAGGGTACAAGCT 50.0 42.9 57.0 56.1 427-446 bp 1.0 59.9
ccsA-ndhD_p2 TTGTGGCTTCTATGGGATTCC TCAGTTGATAGGGTACAAGCT 47.6 42.9 57.7 56.1 428-447 bp 1.0 57.7
ccsA-ndhD_p3 GTGGCTTCTATGGGATTCCT TCAGTTGATAGGGTACAAGCT 50.0 42.9 56.7 56.1 426-445 bp 1.0 57.4
ccsA-ndhD_p4 TGTGGCTTCTATGGGATTCC ATCAGTTGATAGGGTACAAGCT 50.0 40.9 57.0 56.6 428-447 bp 1.0 56.1
ccsA-ndhD_p5 TGTGGCTTCTATGGGATTCCT TCAGTTGATAGGGTACAAGCT 47.6 42.9 58.5 56.1 427-446 bp 1.0 56.0