Hotspot

ndhF-rpl32

SSC · 120262-120929 · 668 bp

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Pi 0.0172

MarkerSeek score 66.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 668
variable_sites: 60
pi: 0.0172
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAAGAAAAGTT	TTTGACATAGAAGTACGTTTTT	40.0	27.3	53.3	52.0	712-800 bp	1.0	50.4
ndhF-rpl32_p2	CTGGAAGTGGAAGAAAAGTT	TTTTGACATAGAAGTACGTTTT	40.0	27.3	53.3	52.0	713-801 bp	1.0	50.4
ndhF-rpl32_p3	CTGGAAGTGGAAGAAAAGTT	TTTTTGACATAGAAGTACGTTT	40.0	27.3	53.3	52.0	714-802 bp	1.0	50.4
ndhF-rpl32_p4	CTGGAAGTGGAAGAAAAGTT	TTTTGACATAGAAGTACGTTTTT	40.0	26.1	53.3	52.9	713-801 bp	1.0	50.4
ndhF-rpl32_p5	CTGGAAGTGGAAGAAAAGTT	TTTTTGACATAGAAGTACGTTTT	40.0	26.1	53.3	52.9	714-802 bp	1.0	50.4