Hotspot

rrn4.5-rrn5

IRb · 112492-112718 · 227 bp

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Pi 0.0347

MarkerSeek score 62.00

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 227
variable_sites: 20
pi: 0.0347
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	344-345 bp	1.0	84.2
rrn4.5-rrn5_p2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	342-343 bp	1.0	84.0
rrn4.5-rrn5_p3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	340-341 bp	1.0	84.0
rrn4.5-rrn5_p4	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	50.0	50.0	58.0	58.0	370-371 bp	1.0	83.9
rrn4.5-rrn5_p5	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	50.0	50.0	58.0	58.0	368-369 bp	1.0	83.7