Hotspot

rbcL-accD

LSC · 60466-61236 · 771 bp

Back to Zingiber

Pi 0.0415

MarkerSeek score 70.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 771
variable_sites: 177
pi: 0.0415
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AATGAGGGACGTGATCTTGC	TTGATTTACTCGGCCCACAC	50.0	50.0	58.0	58.2	984-1005 bp	0.826087	75.5
rbcL-accD_p2	AATGAGGGACGTGATCTTGC	AATTGCACCGCCATTTGAAC	50.0	45.0	58.0	58.2	933-946 bp	0.826087	75.4
rbcL-accD_p3	GTGATCTTGCTCGTGAAGGT	TTGATTTACTCGGCCCACAC	50.0	50.0	57.9	58.2	974-995 bp	0.826087	75.1
rbcL-accD_p4	TTCCAAATGGAGCCCTGAAC	AATTGCACCGCCATTTGAAC	50.0	45.0	58.1	58.2	883-896 bp	0.478261	60.8
rbcL-accD_p5	TTCCAAATGGAGCCCTGAAC	TTGATTTACTCGGCCCACAC	50.0	50.0	58.1	58.2	934-955 bp	0.478261	60.7