Hotspot

ndhC-trnV-UAC

LSC · 53639-55217 · 1579 bp

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Pi 0.00814
MarkerSeek score 62.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1579
variable_sites
74
pi
0.00814
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTAACCGGGGCTAAAACTC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1504-1769 bp 1.0 85.6
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1503-1768 bp 1.0 85.6
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1497-1762 bp 1.0 84.8
ndhC-trnV-UAC_p4 CCTAACCGGGGCTAAAACTC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1586-1851 bp 1.0 83.3
ndhC-trnV-UAC_p5 CTAACCGGGGCTAAAACTCC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1585-1850 bp 1.0 83.3