Hotspot

psbC-trnS-UGA

LSC · 38129-38276 · 148 bp

Back to Zingiber

Pi 0.0412

MarkerSeek score 68.40

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 18
pi: 0.0412
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CTTTCCATGACCCCTCTTCG	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.0	58.0	256-301 bp	1.0	89.9
psbC-trnS-UGA_p2	CATTTGTGGCATGCAGGAAG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.0	58.0	334-379 bp	1.0	88.0
psbC-trnS-UGA_p3	CATTTGTGGCATGCAGGAAG	GCTGAGTGGTTGATAGCTCC	50.0	55.0	58.0	58.1	326-371 bp	1.0	87.9
psbC-trnS-UGA_p4	CATTTGTGGCATGCAGGAAG	GGCTGAGTGGTTGATAGCTC	50.0	55.0	58.0	58.1	327-372 bp	1.0	87.9
psbC-trnS-UGA_p5	CATTTGTGGCATGCAGGAAG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.0	58.1	319-364 bp	1.0	87.8