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trnT-GGU-psbD

LSC · 34417-35697 · 1281 bp

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Pi 0.0134

MarkerSeek score 63.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1281
variable_sites: 115
pi: 0.0134
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGTCTCTGCGTAACCAATCA	55.0	50.0	58.1	57.6	1260-1418 bp	1.0	87.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGTCTCTGCGTAACCAATCA	50.0	50.0	58.1	57.6	1263-1421 bp	1.0	86.9
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GGTCTCTGCGTAACCAATCA	50.0	50.0	58.3	57.6	1237-1395 bp	1.0	85.4
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	GGTCTCTGCGTAACCAATCA	50.0	50.0	57.7	57.6	1275-1433 bp	1.0	85.2
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CGGTCTCTGCGTAACCAATC	55.0	55.0	58.1	58.7	1261-1419 bp	1.0	84.3