Hotspot

petN-psbM

LSC · 30323-31610 · 1288 bp

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Pi 0.0245

MarkerSeek score 69.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1288
variable_sites: 169
pi: 0.0245
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTATGGGGAAGGAGTGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1161-1396 bp	1.0	79.8
petN-psbM_p2	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.1	57.6	1160-1395 bp	1.0	79.8
petN-psbM_p3	ATGGGGAAGGAGTGGACTTT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1158-1393 bp	1.0	78.8
petN-psbM_p4	TGGGGAAGGAGTGGACTTTA	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1157-1392 bp	1.0	77.3
petN-psbM_p5	GTATGGGGAAGGAGTGGACT	TGCTACTGCACTGTTCATTC	55.0	45.0	58.1	56.1	1160-1395 bp	1.0	75.6