Hotspot

ycf1

SSC · 120730-126039 · 5310 bp

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Pi 0.0362
MarkerSeek score 61.30
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5310
variable_sites
285
pi
0.0362
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CTTCTTCAACAGCCCCTTCA AGAAATAAACGGGGCCCAAG 50.0 50.0 57.7 58.2 584-593 bp 0.666667 66.0
ycf1_p2 CTTCTTCAACAGCCCCTTCA GAAATAAACGGGGCCCAAGA 50.0 50.0 57.7 58.2 583-592 bp 0.666667 65.9
ycf1_p3 CTTCTTCAACAGCCCCTTCA AAATAAACGGGGCCCAAGAA 50.0 45.0 57.7 57.7 582-591 bp 0.666667 64.7
ycf1_p4 CTTCTTCAACAGCCCCTTCA AATAAACGGGGCCCAAGAAA 50.0 45.0 57.7 57.7 581-590 bp 0.666667 64.7
ycf1_p5 CTTCTTCAACAGCCCCTTCA TAAACGGGGCCCAAGAAATC 50.0 50.0 57.7 58.2 579 bp 0.333333 49.9