Hotspot

ndhF-rpl32

SSC · 109828-110605 · 778 bp

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Pi 0.086

MarkerSeek score 83.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 778
variable_sites: 94
pi: 0.086
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCCGAAAACCACATAGCTA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	898-1006 bp	1.0	91.4
ndhF-rpl32_p2	ACACATTCCCACAAGTTCCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	1201-1309 bp	1.0	90.3
ndhF-rpl32_p3	CACATTCCCACAAGTTCCCA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.0	58.0	1200-1308 bp	1.0	90.3
ndhF-rpl32_p4	AAGGGGAAATTGTGCGGATT	TCCACCGGACAGTCAAAAAG	45.0	50.0	58.1	58.0	1468-1576 bp	1.0	89.1
ndhF-rpl32_p5	AGGGGAAATTGTGCGGATTT	TCCACCGGACAGTCAAAAAG	45.0	50.0	58.1	58.0	1467-1575 bp	1.0	89.1