Hotspot

accD-psaI

LSC · 56908-57594 · 687 bp

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Pi 0.096

MarkerSeek score 84.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 90
pi: 0.096
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCTACAACTGGCGGAGTTAC	AACGAAGAAAGATGCGACGA	55.0	45.0	58.0	57.9	1649-1784 bp	1.0	88.0
accD-psaI_p2	CCTACAACTGGCGGAGTTAC	CCAGGAAATCCCCAACGAAA	55.0	50.0	58.0	58.1	1623-1758 bp	1.0	87.9
accD-psaI_p3	CCTACAACTGGCGGAGTTAC	CACCGCCTACATTCCATGAA	55.0	50.0	58.0	58.0	1562-1665 bp	0.666667	73.0
accD-psaI_p4	TTGCGTTTACGGGGAAAAGA	CACCGCCTACATTCCATGAA	45.0	50.0	58.0	58.0	1489-1592 bp	0.666667	73.0
accD-psaI_p5	CCTACAACTGGCGGAGTTAC	TCTCTCGCCTCCATTCTGAT	55.0	50.0	58.0	57.9	1692-1795 bp	0.666667	72.2