Hotspot

ycf3-trnS-GGA

LSC · 42187-42781 · 595 bp

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Pi 0.0611
MarkerSeek score 87.90
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
595
variable_sites
54
pi
0.0611
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGGCTACAATTGAAGAGGTCT TTCCAATGCTACGCCTTGAA 42.9 45.0 56.6 57.8 653-693 bp 1.0 70.0
ycf3-trnS-GGA_p2 TGGCTACAATTGAAGAGGTCTT TTCCAATGCTACGCCTTGAA 40.9 45.0 57.4 57.8 653-693 bp 1.0 67.7
ycf3-trnS-GGA_p3 TTGGCTACAATTGAAGAGGTCT TTCCAATGCTACGCCTTGAA 40.9 45.0 57.4 57.8 654-694 bp 1.0 67.7
ycf3-trnS-GGA_p4 TGGCTACAATTGAAGAGGTCT AGTTCCAATGCTACGCCTTG 42.9 50.0 56.6 58.5 655-695 bp 1.0 67.1
ycf3-trnS-GGA_p5 TGGCTACAATTGAAGAGGTCT CAGTTCCAATGCTACGCCTT 42.9 50.0 56.6 58.5 656-696 bp 1.0 67.1