Hotspot

psbC-trnS-UGA

LSC · 33220-33359 · 140 bp

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Pi 0.0657

MarkerSeek score 78.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 13
pi: 0.0657
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GGTTCGAATCCCCCTCTCT	55.0	57.9	58.2	58.1	184-192 bp	1.0	79.1
psbC-trnS-UGA_p2	TTCCATGACCCCTCTTAGCT	GGTTCGAATCCCCCTCTCT	50.0	57.9	57.7	58.1	176-184 bp	1.0	78.6
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	TTCGAATCCCCCTCTCTCC	55.0	57.9	58.2	57.8	182-190 bp	1.0	78.0
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	CAAGGAACTATCGAGGGTTCG	55.0	52.4	58.2	58.2	199-207 bp	1.0	77.5
psbC-trnS-UGA_p5	AGCGGGGTTTGAAAAAGGAA	GGTTCGAATCCCCCTCTCT	45.0	57.9	58.2	58.1	221-229 bp	1.0	77.1