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trnC-GCA-petN

LSC · 26966-27828 · 863 bp

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Pi 0.0457

MarkerSeek score 78.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 863
variable_sites: 58
pi: 0.0457
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGTCAGTTTTTAAGCGACGC	GCTCTAAAGCCCACTCCTTC	50.0	55.0	58.1	58.0	1253-1283 bp	0.666667	68.3
trnC-GCA-petN_p2	GGTCAGTTTTTAAGCGACGC	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.1	57.9	1895-1940 bp	0.666667	67.6
trnC-GCA-petN_p3	TCAAGCACGATGATCCTTGG	GCTCTAAAGCCCACTCCTTC	50.0	55.0	58.0	58.0	1512 bp	0.333333	52.9
trnC-GCA-petN_p4	TCAAGCACGATGATCCTTGG	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.0	57.9	2154 bp	0.333333	52.5
trnC-GCA-petN_p5	CTGGGTGTCGCCTGATAAAA	GCTCTAAAGCCCACTCCTTC	50.0	55.0	57.9	58.0	979 bp	0.333333	52.2