Hotspot

ycf1

SSC · 126931-132309 · 5379 bp

Back to Zephyranthes

Pi 0.00651

MarkerSeek score 44.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5379
variable_sites: 76
pi: 0.00651
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGATTGGTATGATCCACGGT	CCAATACGCATATCGACATCA	45.0	42.9	56.3	56.0	258 bp	1.0	44.9
ycf1_p2	TGATTGGTATGATCCACGGT	CCAATACGCATATCGACATCAAG	45.0	43.5	56.3	57.8	258 bp	1.0	43.1
ycf1_p3	TGATTGGTATGATCCACGGTT	CCAATACGCATATCGACATCA	42.9	42.9	57.1	56.0	258 bp	1.0	43.0
ycf1_p4	TTGATTGGTATGATCCACGGT	CCAATACGCATATCGACATCA	42.9	42.9	57.1	56.0	259 bp	1.0	43.0
ycf1_p5	TGATTGGTATGATCCACGGT	CGCATATCGACATCAAGATTCA	45.0	40.9	56.3	56.8	252 bp	1.0	42.8