Hotspot

ndhD

SSC · 118470-119966 · 1497 bp

Back to Zephyranthes

Pi 0.00401

MarkerSeek score 42.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1497
variable_sites: 13
pi: 0.00401
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGACTTTGCTTTCTGTCTCA	GACAAAAGCCCGTACTCGAT	40.0	50.0	54.5	58.0	1612-1613 bp	1.0	52.9
ndhD_p2	AAGACTTTGCTTTCTGTCTCA	GACAAAAGCCCGTACTCGAT	38.1	50.0	55.4	58.0	1613-1614 bp	1.0	51.6
ndhD_p3	AGACTTTGCTTTCTGTCTCA	TTACCGACAAAAGCCCGTAC	40.0	50.0	54.5	58.2	1617-1618 bp	1.0	51.3
ndhD_p4	AGACTTTGCTTTCTGTCTCA	TTTTACCGACAAAAGCCCGT	40.0	45.0	54.5	58.3	1619-1620 bp	1.0	50.3
ndhD_p5	AAGACTTTGCTTTCTGTCTCA	TTACCGACAAAAGCCCGTAC	38.1	50.0	55.4	58.2	1618-1619 bp	1.0	50.0