Hotspot

rps15-ycf1

SSC · 126556-126930 · 375 bp

Back to Zephyranthes

Pi 0.0149

MarkerSeek score 58.80

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 375
variable_sites: 12
pi: 0.0149
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CTTGCCGAATAGTTGAGGTGA	CGGCAGTCGTTTCAGTATGT	47.6	50.0	58.0	58.3	470-473 bp	1.0	71.7
rps15-ycf1_p2	TCTTGCCGAATAGTTGAGGTG	CGGCAGTCGTTTCAGTATGT	47.6	50.0	58.0	58.3	471-474 bp	1.0	71.7
rps15-ycf1_p3	TGCCGAATAGTTGAGGTGAA	CGGCAGTCGTTTCAGTATGT	45.0	50.0	56.9	58.3	468-471 bp	1.0	70.8
rps15-ycf1_p4	TTGCCGAATAGTTGAGGTGA	CGGCAGTCGTTTCAGTATGT	45.0	50.0	56.9	58.3	469-472 bp	1.0	70.8
rps15-ycf1_p5	TTGCCGAATAGTTGAGGTGAA	CGGCAGTCGTTTCAGTATGT	42.9	50.0	57.6	58.3	469-472 bp	1.0	68.6