Hotspot

ccsA-ndhD

SSC · 118230-118469 · 240 bp

Back to Zephyranthes

Pi 0.0358

MarkerSeek score 59.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 240
variable_sites: 16
pi: 0.0358
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGAATTCGGCACTTGTAGCT	TCAGTTGACAAAGTGCAAGC	50.0	45.0	58.6	57.2	407 bp	1.0	71.2
ccsA-ndhD_p2	CGAATTCGGCACTTGTAGCT	CAGTTGACAAAGTGCAAGCT	50.0	45.0	58.6	57.1	406 bp	1.0	71.2
ccsA-ndhD_p3	ATTCGGCACTTGTAGCTTCT	TCAGTTGACAAAGTGCAAGC	45.0	45.0	57.2	57.2	404 bp	1.0	70.2
ccsA-ndhD_p4	ATTCGGCACTTGTAGCTTCT	CAGTTGACAAAGTGCAAGCT	45.0	45.0	57.2	57.1	403 bp	1.0	70.2
ccsA-ndhD_p5	CGAATTCGGCACTTGTAGCT	CGCTATCAGTTGACAAAGTGC	50.0	47.6	58.6	57.9	412 bp	1.0	69.1