Hotspot

ycf1-ndhF

SSC · 112982-112993 · 12 bp

Back to Zephyranthes

Pi 0.144

MarkerSeek score 65.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 12
variable_sites: 3
pi: 0.144
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACAGGAACAAGAGGGATCCA	TGAAGAAGAGCCCCAAGAGA	50.0	50.0	58.0	58.0	491-768 bp	1.0	83.6
ycf1-ndhF_p2	ACAGGAACAAGAGGGATCCA	GTGAAGAAGAGCCCCAAGAG	50.0	55.0	58.0	57.9	492-769 bp	1.0	83.0
ycf1-ndhF_p3	GGATCCACCGAACAAGACAA	TGAAGAAGAGCCCCAAGAGA	50.0	50.0	57.8	58.0	478-755 bp	1.0	82.6
ycf1-ndhF_p4	ACAGGAACAAGAGGGATCCA	CGAAGGATTCCTCGGATTGG	50.0	55.0	58.0	58.1	635-912 bp	1.0	82.1
ycf1-ndhF_p5	GGATCCACCGAACAAGACAA	GTGAAGAAGAGCCCCAAGAG	50.0	55.0	57.8	57.9	479-756 bp	1.0	82.0