Hotspot

psaJ-rpl33

LSC · 69474-69967 · 494 bp

Back to Zephyranthes

Pi 0.0145

MarkerSeek score 56.20

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 17
pi: 0.0145
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTGGGTCTTTAGCGGGTCTA	GACTCGGCTCTCTCAAACTG	50.0	55.0	58.1	58.0	1188-1215 bp	1.0	83.4
psaJ-rpl33_p2	TTGGGTCTTTAGCGGGTCTA	CCGGCGATTCCTTATTGACA	50.0	50.0	58.1	58.1	647-662 bp	1.0	83.3
psaJ-rpl33_p3	TTGGGTCTTTAGCGGGTCTA	AGTTCTAGGGATCGACTCGG	50.0	55.0	58.1	58.0	1201-1228 bp	1.0	83.2
psaJ-rpl33_p4	ACCCGTGACATTTTGTACCC	GACTCGGCTCTCTCAAACTG	50.0	55.0	58.1	58.0	1658-1696 bp	1.0	83.2
psaJ-rpl33_p5	CCCGTGACATTTTGTACCCA	GACTCGGCTCTCTCAAACTG	50.0	55.0	58.1	58.0	1657-1695 bp	1.0	83.2