Hotspot

petA-psbJ

LSC · 65266-66428 · 1163 bp

Back to Zephyranthes

Pi 0.0184

MarkerSeek score 53.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 45
pi: 0.0184
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	AGTTGATGCATCGGATGGAC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.0	58.0	1448-1463 bp	1.0	83.3
petA-psbJ_p2	AGTTGATGCATCGGATGGAC	TGGGGTCAATATCCGCAATG	50.0	50.0	58.0	58.0	1853-1868 bp	1.0	82.6
petA-psbJ_p3	CAAGATCCATTACGCGTCCA	TGGGGTCAATATCCGCAATG	50.0	50.0	58.1	58.0	1699-1714 bp	1.0	82.6
petA-psbJ_p4	AGTTGATGCATCGGATGGAC	GAGGCCACCAATGACCATAG	50.0	55.0	58.0	58.0	1945-1960 bp	1.0	82.4
petA-psbJ_p5	AGTTGATGCATCGGATGGAC	GAGTTAGGAGGCCACCAATG	50.0	55.0	58.0	58.0	1952-1967 bp	1.0	82.3