Hotspot

atpB-rbcL

LSC · 56542-57493 · 952 bp

Back to Zephyranthes

Pi 0.00903

MarkerSeek score 48.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 952
variable_sites: 18
pi: 0.00903
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCCGACTGGAACACTTAGAG	AGATCCTCCAGACGTAGAGC	55.0	55.0	58.0	58.0	1610-1674 bp	1.0	84.4
atpB-rbcL_p2	GCCGACTGGAACACTTAGAG	ATCCTCCAGACGTAGAGCTC	55.0	55.0	58.0	58.0	1608-1672 bp	1.0	84.4
atpB-rbcL_p3	GCCGACTGGAACACTTAGAG	GATCCTCCAGACGTAGAGCT	55.0	55.0	58.0	58.0	1609-1673 bp	1.0	84.4
atpB-rbcL_p4	GCCGACTGGAACACTTAGAG	GGGGACGACCATACTTGTTC	55.0	55.0	58.0	58.0	1702-1766 bp	1.0	84.2
atpB-rbcL_p5	GCCGACTGGAACACTTAGAG	CGGTCTCTCCAACGCATAAA	55.0	50.0	58.0	58.0	1847-1911 bp	1.0	84.1