Hotspot

trnE-UUC-trnT-GGU

LSC · 32267-32987 · 721 bp

Back to Zephyranthes

Pi 0.00694

MarkerSeek score 58.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 11
pi: 0.00694
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	860-869 bp	1.0	80.1
trnE-UUC-trnT-GGU_p2	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	822-831 bp	1.0	79.3
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	801-810 bp	1.0	79.2
trnE-UUC-trnT-GGU_p4	TTTCGTAGTACCCTACCCCC	CCGATGACTTACGCCTTACC	55.0	55.0	57.8	58.2	857-866 bp	1.0	78.9
trnE-UUC-trnT-GGU_p5	ACTAGACGATGGGGGCATAT	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	790-799 bp	1.0	78.8