Hotspot

trnS-GCU-trnG-UCC

LSC · 8610-9551 · 942 bp

Back to Zephyranthes

Pi 0.0102

MarkerSeek score 48.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 21
pi: 0.0102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	CGAACGAATCACACTTTTACCA	45.0	40.9	58.0	57.3	1019-1281 bp	1.0	60.8
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	TCGAACGAATCACACTTTTACC	45.0	40.9	58.0	57.0	1020-1282 bp	1.0	58.8
trnS-GCU-trnG-UCC_p3	CGGAGAGAGAGGGATTCGAA	CGAACGAATCACACTTTTACCA	55.0	40.9	58.3	57.3	1067-1329 bp	1.0	58.5
trnS-GCU-trnG-UCC_p4	GCTTTAGTCCACTCAGCCAT	CGAACGAATCACACTTTTACCA	50.0	40.9	57.6	57.3	1005-1267 bp	1.0	57.9
trnS-GCU-trnG-UCC_p5	AGTCCACTCAGCCATCTCTC	CGAACGAATCACACTTTTACCA	55.0	40.9	58.5	57.3	1000-1262 bp	1.0	57.1