Hotspot

trnK-UUU-rps16

LSC · 4050-4632 · 583 bp

Back to Zephyranthes

Pi 0.00703
MarkerSeek score 62.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
583
variable_sites
9
pi
0.00703
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCTGGACGAATTCCTTGC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.9 58.2 740-745 bp 1.0 79.6
trnK-UUU-rps16_p2 GTCTGGACGAATTCCTTGCT GGTGCTCAACCTACAGGAAC 50.0 55.0 57.9 58.2 739-744 bp 1.0 79.6
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 684-689 bp 1.0 79.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 683-688 bp 1.0 79.2
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 670-675 bp 1.0 76.9