Hotspot

ycf3

LSC · 44699-46760 · 2062 bp

Back to Zelkova

Pi 0.00535

MarkerSeek score 56.40

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2062
variable_sites: 18
pi: 0.00535
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TGACTGATAGAGACAATAAACA	GTGGGATTCTTTTCTCACGA	31.8	45.0	52.3	55.4	2210-2242 bp	1.0	43.4
ycf3_p2	TGACTGATAGAGACAATAAACAA	GTGGGATTCTTTTCTCACGA	30.4	45.0	53.2	55.4	2210-2242 bp	1.0	43.4
ycf3_p3	TTGACTGATAGAGACAATAAACA	GTGGGATTCTTTTCTCACGA	30.4	45.0	53.2	55.4	2211-2243 bp	1.0	43.4
ycf3_p4	TGACTGATAGAGACAATAAACA	GTGGGATTCTTTTCTCACGAA	31.8	42.9	52.3	56.2	2210-2242 bp	1.0	43.4
ycf3_p5	ACAAATAGAAAACAATTCGAAACT	GTGGGATTCTTTTCTCACGA	25.0	45.0	54.0	55.4	2178-2210 bp	1.0	43.4