Hotspot

atpF

LSC · 12854-14149 · 1296 bp

Back to Zelkova

Pi 0.00296

MarkerSeek score 39.50

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1296
variable_sites: 6
pi: 0.00296
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TGCTCGAATGGTTACCATGA	AGTCTATCTATAAGAAAGAGGAGATCA	45.0	33.3	56.9	56.5	1404-1417 bp	1.0	40.6
atpF_p2	GCTCGAATGGTTACCATGAGT	AGTCTATCTATAAGAAAGAGGAGATCA	47.6	33.3	58.1	56.5	1403-1416 bp	1.0	40.6
atpF_p3	CTGCTCGAATGGTTACCATGA	AGTCTATCTATAAGAAAGAGGAGATCA	47.6	33.3	58.1	56.5	1405-1418 bp	1.0	40.6
atpF_p4	TCTGCTCGAATGGTTACCATG	AGTCTATCTATAAGAAAGAGGAGATCA	47.6	33.3	58.1	56.5	1406-1419 bp	1.0	40.6
atpF_p5	TGCTCGAATGGTTACCATGAG	AGTCTATCTATAAGAAAGAGGAGATCA	47.6	33.3	58.1	56.5	1404-1417 bp	1.0	40.6