Hotspot

rps11-rpl36

LSC · 82620-82754 · 135 bp

Back to Zelkova

Pi 0.016

MarkerSeek score 63.40

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 4
pi: 0.016
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CTCCCTTTGGTATTCTACGCA	ATCCGTAGACGAGGACGAAT	47.6	50.0	57.5	58.0	286 bp	1.0	68.5
rps11-rpl36_p2	ACTCCCTTTGGTATTCTACGC	ATCCGTAGACGAGGACGAAT	47.6	50.0	57.5	58.0	287 bp	1.0	68.5
rps11-rpl36_p3	CTCCCTTTGGTATTCTACGCA	GACTGATCCGTAGACGAGGA	47.6	55.0	57.5	58.0	291 bp	1.0	68.4
rps11-rpl36_p4	ACTCCCTTTGGTATTCTACGC	GACTGATCCGTAGACGAGGA	47.6	55.0	57.5	58.0	292 bp	1.0	68.4
rps11-rpl36_p5	TCCCTTTGGTATTCTACGCA	ATCCGTAGACGAGGACGAAT	45.0	50.0	56.2	58.0	285 bp	1.0	66.8