Hotspot

trnT-GGU-psbD

LSC · 33646-34830 · 1185 bp

Back to Zelkova

Pi 0.0104
MarkerSeek score 67.30
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1185
variable_sites
20
pi
0.0104
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAGCAATAGACCAGACCAGC 55.0 55.0 58.1 58.1 1349-1410 bp 1.0 81.3
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAGCAATAGACCAGACCAGC 50.0 55.0 58.1 58.1 1352-1413 bp 1.0 81.1
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1327-1388 bp 1.0 79.8
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1330-1391 bp 1.0 79.6
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG AGACCAGACCAGCCTACAAA 55.0 50.0 58.1 58.3 1342-1403 bp 1.0 79.6