Hotspot

rps2-rpoC2

LSC · 17326-17545 · 220 bp

Back to Zelkova

Pi 0.00758
MarkerSeek score 60.90
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
220
variable_sites
3
pi
0.00758
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 GGTGCCATTCTAGGATTCCA TTCGAGTGGGAAATCAGAGA 50.0 45.0 57.0 55.9 435-442 bp 1.0 56.7
rps2-rpoC2_p2 TGACCAAAATGAACTCCTGCT TTCGAGTGGGAAATCAGAGA 42.9 45.0 57.8 55.9 402-409 bp 1.0 55.3
rps2-rpoC2_p3 ACTCCTGCTTCCATCATCTCT TTCGAGTGGGAAATCAGAGA 47.6 45.0 58.2 55.9 390-397 bp 1.0 55.3
rps2-rpoC2_p4 TGAACTCCTGCTTCCATCATC TTCGAGTGGGAAATCAGAGA 47.6 45.0 57.7 55.9 393-400 bp 1.0 55.0
rps2-rpoC2_p5 GGTGCCATTCTAGGATTCCA TCGAGTGGGAAATCAGAGAT 50.0 45.0 57.0 55.7 434-441 bp 1.0 54.8