Hotspot

rps16-trnQ-UUG

LSC · 6465-7549 · 1085 bp

Back to Zelkova

Pi 0.00646

MarkerSeek score 62.20

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 11
pi: 0.00646
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1105-1175 bp	1.0	83.5
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1088-1158 bp	1.0	83.1
rps16-trnQ-UUG_p3	AACGGATCGTGTCCTTCAAG	TAAGGCATCGGGTTTTGGTC	50.0	50.0	57.9	58.2	1135-1205 bp	1.0	82.8
rps16-trnQ-UUG_p4	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1106-1176 bp	1.0	82.5
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TAAGGCATCGGGTTTTGGTC	45.0	50.0	58.1	58.2	1118-1188 bp	1.0	82.3