Hotspot

trnH-GUG-psbA

LSC · 75-272 · 198 bp

Back to Zelkova

Pi 0.053

MarkerSeek score 83.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 15
pi: 0.053
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	AATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.7	57.9	264-323 bp	1.0	83.9
trnH-GUG-psbA_p2	AATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.7	57.9	267-326 bp	1.0	83.7
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.7	58.0	314-373 bp	1.0	83.1
trnH-GUG-psbA_p4	TTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	45.0	55.0	57.4	57.9	271-330 bp	1.0	81.5
trnH-GUG-psbA_p5	TTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	45.0	50.0	57.4	58.0	321-380 bp	1.0	80.8