Hotspot

ycf1

SSC · 126776-132286 · 5511 bp

Back to Zehneria

Pi 0.0107
MarkerSeek score 46.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5511
variable_sites
152
pi
0.0107
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TAAGGTCCGCTCAACAACG TGGAATTATCTCAATGAACAAAA 52.6 26.1 57.8 52.6 188 bp 1.0 44.3
ycf1_p2 CGATAAGGTCCGCTCAACAA TGGAATTATCTCAATGAACAAAA 50.0 26.1 58.0 52.6 191 bp 1.0 44.2
ycf1_p3 CGATAAGGTCCGCTCAACAA TGGAATTATCTCAATGAACAAAAA 50.0 25.0 58.0 53.4 191 bp 1.0 44.2
ycf1_p4 ATAAGGTCCGCTCAACAACG TGGAATTATCTCAATGAACAAAA 50.0 26.1 58.3 52.6 189 bp 1.0 44.2
ycf1_p5 ATAAGGTCCGCTCAACAACG TGGAATTATCTCAATGAACAAAAA 50.0 25.0 58.3 53.4 189 bp 1.0 44.2