Hotspot

trnR-trnN

IRb · 110951-111567 · 617 bp

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Pi 0.00648

MarkerSeek score 58.30

Primer available yes

Label trnR-trnN

Feature Metadata

label_name: trnR-trnN
feature_type: igs
region: IRb
length_bp: 617
variable_sites: 10
pi: 0.00648
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnN_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	760 bp	1.0	79.3
trnR-trnN_p2	GAACAGATCCAGTGGAGACG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.7	58.0	797 bp	1.0	78.7
trnR-trnN_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	711 bp	1.0	78.1
trnR-trnN_p4	CAGAGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	55.0	50.0	57.8	57.8	800 bp	1.0	77.7
trnR-trnN_p5	GAACAGATCCAGTGGAGACG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.7	58.2	748 bp	1.0	77.5