Hotspot

trnF-ndhJ

LSC · 50861-51562 · 702 bp

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Pi 0.0116

MarkerSeek score 66.40

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 19
pi: 0.0116
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGGTAGGATGGCC	50.0	55.0	58.1	58.2	806-812 bp	1.0	80.2
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGTAGGATGGCCCTTACGTA	50.0	55.0	58.1	58.3	798-804 bp	1.0	79.7
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGGTAGGATGGC	50.0	50.0	58.1	57.5	807-813 bp	1.0	78.0
trnF-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCTGAAAGTTGGGTAGG	50.0	50.0	58.1	57.5	812-818 bp	1.0	77.9
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGCCTGAAAGTTGGGTAGGA	50.0	50.0	58.1	58.6	811-817 bp	1.0	77.6