Hotspot

psbM-trnD

LSC · 31145-32252 · 1108 bp

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Pi 0.0136

MarkerSeek score 58.40

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 1108
variable_sites: 36
pi: 0.0136
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	AGGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1234-1235 bp	1.0	70.7
psbM-trnD_p2	AGGCGGTAGGAACTAGAATGA	GTTCAATTGGTCAGAGCACC	47.6	50.0	58.0	57.0	1235-1236 bp	1.0	66.6
psbM-trnD_p3	GGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.4	58.5	1233-1234 bp	1.0	66.5
psbM-trnD_p4	AGGCGGTAGGAACTAGAATG	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.4	58.5	1234-1235 bp	1.0	66.4
psbM-trnD_p5	AGGCGGTAGGAACTAGAATGA	TCAATTGGTCAGAGCACCG	47.6	52.6	58.0	57.8	1233-1234 bp	1.0	64.7