Hotspot

petN-psbM

LSC · 30138-31039 · 902 bp

Back to Zehneria

Pi 0.013

MarkerSeek score 58.70

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 902
variable_sites: 30
pi: 0.013
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TTCCCTCGTAGTATGGGGAC	TCATTCTAGTTCCTACCGCCT	55.0	47.6	57.9	58.0	984-1011 bp	1.0	75.2
petN-psbM_p2	CCTCGTAGTATGGGGACGAA	TCATTCTAGTTCCTACCGCCT	55.0	47.6	58.3	58.0	981-1008 bp	1.0	73.3
petN-psbM_p3	TTCCCTCGTAGTATGGGGAC	TGCTACTGCACTGTTCATTCT	55.0	42.9	57.9	57.6	998-1025 bp	1.0	72.1
petN-psbM_p4	TTCCCTCGTAGTATGGGGAC	TCATTCTAGTTCCTACCGCC	55.0	50.0	57.9	56.4	984-1011 bp	1.0	70.9
petN-psbM_p5	TTCCCTCGTAGTATGGGGAC	CATTCTAGTTCCTACCGCCT	55.0	50.0	57.9	56.4	983-1010 bp	1.0	70.8