Hotspot

trnC-petN

LSC · 29155-30047 · 893 bp

Back to Zehneria

Pi 0.0109

MarkerSeek score 59.90

Primer available yes

Label trnC-petN

Feature Metadata

label_name: trnC-petN
feature_type: igs
region: LSC
length_bp: 893
variable_sites: 23
pi: 0.0109
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-petN_p1	TTTCCCCAGTTCAAATCCGG	TCTAGAGTCCACTTCGTCCC	50.0	55.0	58.1	57.9	1004-1014 bp	1.0	82.0
trnC-petN_p2	TGCAAATCCCTTTTTCCCCA	TCTAGAGTCCACTTCGTCCC	45.0	55.0	57.9	57.9	1016-1026 bp	1.0	81.7
trnC-petN_p3	TTTCCCCAGTTCAAATCCGG	TTCGTCCCCATACTACGAGG	50.0	55.0	58.1	58.3	992-1002 bp	1.0	80.5
trnC-petN_p4	TGCAAATCCCTTTTTCCCCA	TTCGTCCCCATACTACGAGG	45.0	55.0	57.9	58.3	1004-1014 bp	1.0	80.2
trnC-petN_p5	TTTCCCCAGTTCAAATCCGG	ACTTCGTCCCCATACTACGA	50.0	50.0	58.1	57.6	994-1004 bp	1.0	79.6