Hotspot

trnR-atpA

LSC · 10776-11252 · 477 bp

Back to Zehneria

Pi 0.0227

MarkerSeek score 66.90

Primer available yes

Label trnR-atpA

Feature Metadata

label_name: trnR-atpA
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 29
pi: 0.0227
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-atpA_p1	TGGAATGAAAAGCGTCCATTG	TCCACGAAGACATTCACCAA	42.9	45.0	57.4	57.1	613-755 bp	1.0	67.3
trnR-atpA_p2	TGGAATGAAAAGCGTCCATTG	TTCCACGAAGACATTCACCA	42.9	45.0	57.4	57.1	614-756 bp	1.0	67.3
trnR-atpA_p3	GGAATGAAAAGCGTCCATTGT	TCCACGAAGACATTCACCAA	42.9	45.0	57.4	57.1	612-754 bp	1.0	67.3
trnR-atpA_p4	GGAATGAAAAGCGTCCATTGT	TTCCACGAAGACATTCACCA	42.9	45.0	57.4	57.1	613-755 bp	1.0	67.3
trnR-atpA_p5	TGGAATGAAAAGCGTCCATTG	GGAAGCCCTTTTGAAAGAAGC	42.9	47.6	57.4	58.0	587-729 bp	1.0	66.6