Hotspot

rpl16

LSC · 79849-81314 · 1466 bp

Pi 0.00268

MarkerSeek score 49.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1466
variable_sites: 13
pi: 0.00268
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAGAAAGAAAAACCAGGGG	ACCTTGTTTTTCCAAAAATCCCT	47.6	34.8	58.0	57.3	80-1613 bp	1.0	43.6
rpl16_p2	CCAGAAAGAAAAACCAGGGGT	ACCTTGTTTTTCCAAAAATCCCT	47.6	34.8	58.3	57.3	80-1612 bp	1.0	41.6
rpl16_p3	TCCAGAAAGAAAAACCAGGGG	AGACAAGAACCTTGTTTTTCCA	47.6	36.4	58.0	56.3	80-1621 bp	0.857143	36.7
rpl16_p4	CCAGAAAGAAAAACCAGGGGT	AGACAAGAACCTTGTTTTTCCA	47.6	36.4	58.3	56.3	80-1620 bp	0.857143	34.6
rpl16_p5	TCCAGAAAGAAAAACCAGGGG	AGACAAGAACCTTGTTTTTCCAA	47.6	34.8	58.0	57.0	80-1621 bp	0.857143	34.6