Hotspot

psbD

LSC · 9355-10416 · 1062 bp

Pi 0.0043

MarkerSeek score 34.90

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 16
pi: 0.0043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	AGGTGCTCGAAAATGGTTGA	GTCACGACCAGCTAAAACGA	45.0	50.0	57.7	58.2	1133 bp	1.0	77.2
psbD_p2	TGAGGTGCTCGAAAATGGTT	GTCACGACCAGCTAAAACGA	45.0	50.0	57.7	58.2	1135 bp	1.0	77.2
psbD_p3	ATGAGGTGCTCGAAAATGGT	GTCACGACCAGCTAAAACGA	45.0	50.0	57.5	58.2	1136 bp	1.0	75.3
psbD_p4	AGGTGCTCGAAAATGGTTGA	TCACGACCAGCTAAAACGAA	45.0	45.0	57.7	57.5	1132 bp	1.0	74.9
psbD_p5	TGAGGTGCTCGAAAATGGTT	TCACGACCAGCTAAAACGAA	45.0	45.0	57.7	57.5	1134 bp	1.0	74.9