Hotspot

rpl32-trnL-UAG

SSC · 108643-109173 · 531 bp

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Pi 0.00377
MarkerSeek score 47.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
531
variable_sites
4
pi
0.00377
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ATTTTCTAGGGGCAACGAGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 80-634 bp 1.0 79.8
rpl32-trnL-UAG_p2 ATTTTCTAGGGGCAACGAGC AGCAGCGTGTCTACCAATTT 50.0 45.0 58.2 57.8 80-627 bp 1.0 79.3
rpl32-trnL-UAG_p3 ATTTTCTAGGGGCAACGAGC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.2 58.0 80-661 bp 0.857143 74.7
rpl32-trnL-UAG_p4 ATTTTCTAGGGGCAACGAGC GGATTCGAACCGAGATGCTT 50.0 50.0 58.2 58.1 80-664 bp 0.857143 74.3
rpl32-trnL-UAG_p5 GGTTTTTCTGGGCAACAAGC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.4 58.0 80-627 bp 0.857143 73.5