Hotspot

petA-psbJ

LSC · 62890-63801 · 912 bp

Pi 0.00428

MarkerSeek score 38.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 912
variable_sites: 8
pi: 0.00428
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 38.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGCCTTTTGTTTT	ACGGTTCGTATTCTGGATTGG	45.0	47.6	57.4	58.1	80-1057 bp	1.0	68.4
petA-psbJ_p2	CGTCCAAGGCCTTTTGTTTT	GGTTCGTATTCTGGATTGGGT	45.0	47.6	57.4	57.7	80-1055 bp	1.0	67.0
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTTTT	ACGGTTCGTATTCTGGATTGG	42.9	47.6	58.1	58.1	80-1057 bp	1.0	64.3
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTTT	CGGTTCGTATTCTGGATTGG	45.0	50.0	57.4	56.3	80-1056 bp	1.0	63.7
petA-psbJ_p5	AGGCCTTTTGTTTTTCTTCGC	ACGGTTCGTATTCTGGATTGG	42.9	47.6	58.2	58.1	80-1051 bp	1.0	63.7